The PEG-responding desiccome of the alder microsymbiont Frankia alni.
Kais GhediraEmna Harigua-SouiaiCherif Ben HamdaPascale FournierPetar PujicSihem GuesmiIkram GuizaniGuylaine MiotelloJean ArmengaudPhilippe NormandHaïtham SghaierPublished in: Scientific reports (2018)
Actinorhizal plants are ecologically and economically important. Symbiosis with nitrogen-fixing bacteria allows these woody dicotyledonous plants to colonise soils under nitrogen deficiency, water-stress or other extreme conditions. However, proteins involved in xerotolerance of symbiotic microorganisms have yet to be identified. Here we characterise the polyethylene glycol (PEG)-responding desiccome from the most geographically widespread Gram-positive nitrogen-fixing plant symbiont, Frankia alni, by next-generation proteomics, taking advantage of a Q-Exactive HF tandem mass spectrometer equipped with an ultra-high-field Orbitrap analyser. A total of 2,052 proteins were detected and quantified. Under osmotic stress, PEG-grown F. alni cells increased the abundance of envelope-associated proteins like ABC transporters, mechano-sensitive ion channels and Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated (cas) components. Conjointly, dispensable pathways, like nitrogen fixation, aerobic respiration and homologous recombination, were markedly down-regulated. Molecular modelling and docking simulations suggested that the PEG is acting on Frankia partly by filling the inner part of an up-regulated osmotic-stress large conductance mechanosensitive channel.
Keyphrases
- drug delivery
- high resolution
- crispr cas
- mass spectrometry
- genome editing
- dna damage
- molecular dynamics
- induced apoptosis
- dna repair
- stress induced
- heavy metals
- cell cycle arrest
- minimally invasive
- genome wide
- climate change
- heat stress
- oxidative stress
- molecular dynamics simulations
- endoplasmic reticulum stress
- ultra high performance liquid chromatography
- signaling pathway
- gene expression
- cell death
- tandem mass spectrometry
- ms ms
- high resolution mass spectrometry
- single molecule
- cell proliferation
- high intensity
- microbial community
- replacement therapy
- wastewater treatment