Prenatal Alcohol Exposure in Rats Diminishes Postnatal Cxcl16 Chemokine Ligand Brain Expression.
Pedro Juárez-RodríguezJuliana Marisol Godínez-RubíCarolina Guzmán-BrambilaEdgar Padilla-VelardeArturo Orozco-BarocioDaniel Ortuño-SahagúnArgelia E Rojas-MayorquínPublished in: Brain sciences (2020)
Maternal ethanol consumption during pregnancy is one of the main causes of Neurodevelopmental disorders (NDD). Prenatal alcohol exposure (PAE) produces several adverse manifestations. Even low or moderate intake has been associated with long-lasting behavioral and cognitive impairment in offspring. In this study we examined the gene expression profile in the rat nucleus accumbens using microarrays, comparing animals exposed prenatally to ethanol and controls. Microarray gene expression showed an overall downward regulatory effect of PAE. Gene cluster analysis reveals that the gene groups most affected are related to transcription regulation, transcription factors and homeobox genes. We focus on the expression of the C-X-C motif chemokine ligand 16 (Cxcl16) which was differentially expressed. There is a significant reduction in the expression of this chemokine throughout the brain under PAE conditions, evidenced here by quantitative polymerase chain reaction qPCR and immunohistochemistry. Chemokines are involved in neuroprotection and implicated in alcohol-induced brain damage and neuroinflammation in the developing central nervous system (CNS), therefore, the significance of the overall decrease in Cxcl16 expression in the brain as a consequence of PAE may reflect a reduced ability in neuroprotection against subsequent conditions, such as excitotoxic damage, inflammatory processes or even hypoxic-ischemic insult.
Keyphrases
- poor prognosis
- cerebral ischemia
- gene expression
- transcription factor
- resting state
- white matter
- cognitive impairment
- genome wide
- genome wide identification
- copy number
- functional connectivity
- brain injury
- pregnant women
- dna methylation
- preterm infants
- long non coding rna
- type diabetes
- traumatic brain injury
- alcohol consumption
- blood brain barrier
- insulin resistance
- multiple sclerosis
- physical activity
- high glucose
- subarachnoid hemorrhage
- lps induced
- adipose tissue
- weight loss
- skeletal muscle
- inflammatory response
- drug induced
- endothelial cells
- pregnancy outcomes