Novel Hyp-Gly-containing antiplatelet peptides from collagen hydrolysate after simulated gastrointestinal digestion and intestinal absorption.

Bioactive components causing the antiplatelet activity upon collagen hydrolysate (CH) ingestion have not been clarified yet. This study aimed to identify antiplatelet peptides from CH after simulated gastrointestinal digestion and intestinal absorption. Four antiplatelet peptides containing the Hyp-Gly (OG) sequence including OG, Hyp-Gly-Glu (OGE), Pro-Gly-Glu-Hyp-Gly (PGEOG) and Val-Gly-Pro-Hyp-Gly-Pro-Ala (VGPOGPA) were successfully identified. All four peptides exhibited antiplatelet activity, but OGE and PGEOG exerted stronger activity than OG and VGPOGPA. The IC50 value of OGE and PGEOG was 1.076 mM and 1.167 mM, respectively. These four antiplatelet peptides could survive simulated gastrointestinal digestion and be absorbed intact by Caco-2 cells. Furthermore, plasma stability experiments showed that OG and OGE showed a good stability in human plasma, but PGEOG and VGPOGPA showed a relatively poor stability. In vivo studies indicated that OG and OGE were present in blood after the oral administration of CH. Meanwhile, OGE exerted significant in vivo anti-thrombotic activity after its ingestion. The present study clarifies the antiplatelet components causing the CH activity and highlights the potential application of CH or these four peptides as functional foods to combat thrombosis by inhibiting platelet aggregation.