An integrated toolkit for human microglia functional genomics.
Imdadul HaqJason C NgoNainika RoyRichard L PanNadiya NawsheenRebecca ChiuYa ZhangMasashi FujitaRajesh K SoniXuebing WuDavid A BennettVilas MenonMarta OlahFalak SherPublished in: Stem cell research & therapy (2024)
Our advanced two-step protocol rapidly and efficiently produces authentic iMG. With features like the CRISPR-ON/OFF system and a comprehensive multi-omic data platform, our toolkit equips researchers for robust microglial functional genomic studies. By facilitating detailed SNV investigation and offering a sustainable cell harvest mechanism, the toolkit heralds significant progress in neurodegenerative disease drug research and therapeutic advancement.
Keyphrases
- single cell
- inflammatory response
- endothelial cells
- neuropathic pain
- crispr cas
- high throughput
- genome wide
- electronic health record
- genome editing
- cell therapy
- lipopolysaccharide induced
- copy number
- big data
- induced pluripotent stem cells
- gene expression
- emergency department
- case control
- bone marrow
- machine learning
- data analysis