SLAPSHOT reveals rapid dynamics of extracellularly exposed proteome in response to calcium-activated plasma membrane phospholipid scrambling.

Sami T Tuomivaara Chin Fen Teo Yuh Nung Jan Lily Yeh Jan Arun P Wiita

Published in: bioRxiv : the preprint server for biology (2023)

An enzyme-driven method to tag extracellularly exposed proteins in an unbiased manner with a superior combination of temporal resolution, spatial specificity, and sensitivityA general approach applicable to primary and scarce cells without involving cellular engineeringShort time scale proteome dynamics of Jurkat cells with and without TMEM16F revealed by SLAPSHOT coupled with quantitative mass spectrometry provide insights into phospholipid scrambling-mediated plasma membrane remodeling.

Keyphrases

induced apoptosis
cell cycle arrest
mass spectrometry
high resolution
endoplasmic reticulum stress
fatty acid
cell death
oxidative stress
single molecule
pi k akt
high performance liquid chromatography
liquid chromatography
gas chromatography