Concentration of urine samples improves sensitivity in detection of Strongyloides-specific IgG antibody in urine for diagnosis of strongyloidiasis.

Detection of IgG in urine is an efficient method comparable to that in serum for diagnosis of strongyloidiasis but effects of daily variation in urine dilution on diagnostic accuracy is not clearly known. This study evaluated effects of urine concentration on detection of parasite-specific IgG by urine enzyme-linked immunosorbent assay (ELISA), particularly in individuals with border-line results or false-negative diagnosis. Optimal concentration conditions were established by comparing Strongyloides-specific IgG antibody levels between unconcentrated and concentrated urine in participants with different infection intensities, namely healthy control (HC), low-negative (LN), high-negative (HN) and low-positive (LP) groups. The optimal condition was selected and validated in a field-trial study. The final urine concentration protocol required centrifugation at 4,000 g at 4°C for 10 mins using the Amicon® concentrator tube. This protocol was validated in groups of participants with varying diagnoses according to urine ELISA and fecal examination (n=148). The concentrated-urine ELISA increased the proportion of positive results in the LN group by 68.2% and by 100% in the HN group. Significantly elevated IgG antibody levels were seen in the LP group. In the group that was false negative by urine ELISA but positive by fecal examination (n=28), concentrated-urine ELISA yielded 100% positive results. Overall, the frequency estimates of S. stercoralis were 23.6% by fecal culture, 27% by standard urine ELISA and 90.5% by concentrated-urine ELISA. Concentration of urine samples prior to analysis by ELISA improved the sensitivity for diagnosis and is potentially useful in diagnosis of strongyloidiasis in immunocompromised individuals or in low-prevalence areas.