High-Precision Quantitation of Biofluid Samples Using Direct Mass Spectrometry Analysis.
Wenpeng ZhangYue RenZiqing LinZheng OuyangPublished in: Analytical chemistry (2019)
The transition of mass spectrometry for clinical analysis is highly desirable, and major progress has been made with direct sampling ionization for operation simplification. High-precision quantitation, however, remains a major challenge in this transition. Herein, a novel method was developed for direct quantitation of biofluid samples, using an extremely simplified procedure for incorporation of internal standards selected against the traditional rules. Slug flow microextraction was used for the development, with conditions predicted by a theoretical model, viz., using internal standards of partition coefficients very different from the analytes and large sample-to-extraction solvent volume ratios. Direct quantitation of drug compounds in urine and blood samples was demonstrated. This development enabled an extremely simplified protocol that is expected to have a significant impact on on-site or clinical analysis.
Keyphrases
- mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- gas chromatography
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- ms ms
- solid phase extraction
- simultaneous determination
- capillary electrophoresis
- high resolution
- randomized controlled trial
- emergency department
- signaling pathway