Transcriptome analysis of virulence gene regulation by the ATP-dependent Lon protease in Salmonella Typhimurium.
Xiaorui SongHuan ZhangShuangshuang MaYajun SongRunxia LvXiaoqian LiuBin YangDi HuangBin LiuLingyan JiangPublished in: Future microbiology (2019)
Aim: Determination of the virulence regulatory network controlled by the ATP-dependent Lon protease in Salmonella enterica serovar Typhimurium. Materials & methods: The effect of Lon on S. Typhimurium virulence genes expression was investigated by RNA sequencing, and virulence-associated phenotypes between the wild-type and lon mutant were compared. Results: SPI-1, SPI-4, SPI-9 and flagellar genes were activated, while SPI-2 genes were repressed in the lon mutant. Accordingly, the lon mutant exhibited increased adhesion to and invasion of epithelial cells, increased motility and decreased replication in macrophages. The activation of SPI-2 genes by Lon partially accounts for the replication defect of the mutant. Conclusion: A wide range of virulence regulatory functions are governed by Lon in S. enterica ser. Typhimurium.
Keyphrases
- wild type
- biofilm formation
- escherichia coli
- listeria monocytogenes
- pseudomonas aeruginosa
- antimicrobial resistance
- staphylococcus aureus
- genome wide
- genome wide identification
- candida albicans
- cystic fibrosis
- poor prognosis
- genome wide analysis
- single cell
- long non coding rna
- binding protein
- atomic force microscopy