Improving CRISPR-Cas9 directed faithful transgene integration outcomes by reducing unwanted random DNA integration.
Rio HermantaraLaura RichmondAqeel Faisal TaqiSabari ChilakaValentine JeantetIleana GuerriniKatherine WestAdam WestPublished in: Journal of biomedical science (2024)
Our results highlight the need for a more stringent assessment of CRISPR-Cas9-mediated knock-in outcomes, and the importance of careful strategy design to maximise efficient and faithful transgene integration.