mScarlet3: a brilliant and fast-maturing red fluorescent protein.
Theodorus W J GadellaLaura van WeerenJente StouthamerMark A HinkAnouk H G WoltersBen N G GiepmansSylvain AumonierJerome DupuyAntoine RoyantPublished in: Nature methods (2023)
We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems.
Keyphrases
- energy transfer
- quantum dots
- crystal structure
- living cells
- protein protein
- fluorescent probe
- binding protein
- poor prognosis
- label free
- amino acid
- crispr cas
- single molecule
- dengue virus
- magnetic resonance imaging
- zika virus
- small molecule
- ionic liquid
- contrast enhanced
- computed tomography
- long non coding rna
- subarachnoid hemorrhage
- cerebral ischemia
- diffusion weighted imaging