Mapping protein states and interactions across the tree of life with co-fractionation mass spectrometry.
Michael A SkinniderMopelola O AkinlajaLeonard J FosterPublished in: Nature communications (2023)
We present CFdb, a harmonized resource of interaction proteomics data from 411 co-fractionation mass spectrometry (CF-MS) datasets spanning 21,703 fractions. Meta-analysis of this resource charts protein abundance, phosphorylation, and interactions throughout the tree of life, including a reference map of the human interactome. We show how large-scale CF-MS data can enhance analyses of individual CF-MS datasets, and exemplify this strategy by mapping the honey bee interactome.
Keyphrases
- mass spectrometry
- high resolution
- cystic fibrosis
- liquid chromatography
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- high density
- electronic health record
- endothelial cells
- protein protein
- big data
- rna seq
- amino acid
- binding protein
- multiple sclerosis
- data analysis
- machine learning
- microbial community
- small molecule
- single cell
- artificial intelligence