Mesoporous Silica Promotes Osteogenesis of Human Adipose-Derived Stem Cells Identified by a High-Throughput Microfluidic Chip Assay.
Xin ChenChao WangMin HaoHang ZhaoHe XiaLiyang YuDong LiJichuan QiuHaijun LiLin HanYuanhua SangPublished in: Pharmaceutics (2022)
Silicon-derived biomaterials are conducive to regulating the fate of osteo-related stem cells, while their effects on the osteogenic differentiation of human adipose-derived stem cells (hADSCs) remain inconclusive. Mesoporous silica (mSiO 2 ) is synthesized in a facile route that exhibited the capability of promoting osteogenic differentiation of hADSCs. The metabolism of SiO 2 in cells is proposed according to the colocalization fluorescence analysis between lysosomes and nanoparticles. The released silicon elements promote osteogenic differentiation. The detection of secretory proteins through numerous parallel experiments performed via a microfluidic chip confirms the positive effect of SiO 2 on the osteogenic differentiation of hADSCs. Moreover, constructed with superparamagnetic iron oxide (Fe 3 O 4 ), the magnetic nanoparticles (MNPs) of Fe 3 O 4 @mSiO 2 endow the cells with magnetic resonance imaging (MRI) properties. The MNP-regulated osteogenic differentiation of autologous adipose-derived stem cells provides considerable clinical application prospects for stem cell therapy of bone tissue repair with an effective reduction in immune rejection.
Keyphrases
- high throughput
- cell therapy
- magnetic nanoparticles
- stem cells
- magnetic resonance imaging
- induced apoptosis
- iron oxide
- endothelial cells
- single cell
- circulating tumor cells
- mesenchymal stem cells
- cell cycle arrest
- bone marrow
- induced pluripotent stem cells
- computed tomography
- contrast enhanced
- wound healing
- pluripotent stem cells
- cell death
- single molecule
- diffusion weighted imaging
- label free
- loop mediated isothermal amplification
- endoplasmic reticulum stress
- platelet rich plasma