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Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation.
Celine Everaert
Jasper Verwilt
Kimberly Verniers
Niels Vandamme
Alvaro Marcos Rubio
Jo Vandesompele
Pieter Mestdagh
Published in:
Biological procedures online (2023)
Our method does not require modifications of the library preparation procedure apart from simply adding blocking oligonucleotides to the RT reaction and can thus be easily integrated into virtually any RNA sequencing library preparation protocol.
Keyphrases
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molecularly imprinted
single cell
nucleic acid
randomized controlled trial
rna seq
gene expression
minimally invasive
genome wide
dna methylation
simultaneous determination