Human periodontal ligament stem cells with distinct osteogenic potential induce bone formation in rat calvaria defects.
Leticia Faustino AdolphoHelena Bacha LopesDenise WeffortGabriela Guaraldo Campos TotoliAna Carolina Loyola BarbosaRahyza Inacio Freire AssisKarina Gonzalez Silvério RuizDenise Carleto AndiaAdalberto Luiz RosaAdalberto Luiz RosaPublished in: Regenerative medicine (2022)
Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition to mixed cells, to repair bone tissue. Methods: Cell phenotype, proliferation and differentiation were evaluated. Undifferentiated PDLSCs were injected into rat calvarial defects and the new bone was evaluated by μCT, histology and real-time PCR. Results: PDLSCs exhibited a typical mesenchymal stem cell phenotype and HP-PDLSCs showed lower proliferative and higher osteogenic potential than LP-PDLSCs. PDLSCs induced similar bone formation and histological analysis suggests a remodeling process, confirmed by osteogenic and osteoclastogenic markers, especially in tissues derived from defects treated with HP-PDLSCs. Conclusion: PDLSCs induced similar bone formation irrespective of their in vitro osteogenic potential.
Keyphrases
- mesenchymal stem cells
- stem cells
- bone marrow
- endothelial cells
- cell therapy
- umbilical cord
- oxidative stress
- human health
- signaling pathway
- diabetic rats
- gene expression
- risk assessment
- induced pluripotent stem cells
- single cell
- drug induced
- pluripotent stem cells
- body composition
- cell cycle arrest
- cell death
- dual energy
- stress induced