Selected microRNA Expression and Protein Regulator Secretion by Adipose Tissue-Derived Mesenchymal Stem Cells and Metabolic Syndrome.
Grzegorz WystrychowskiKlaudia Simka-LampaAgnieszka WitkowskaEwelina SobeckoAleksandra Skubis-SikoraBartosz SikoraEwa WojtynaAgnieszka GoldaKatarzyna GwizdekMarta WróbelŁukasz SędekSylwia Górczyńska-KosiorzNikola Szweda-GandorWanda TrautsoltTomasz FrancuzCelina Kruszniewska-RajsJoanna Magdalena GolaPublished in: International journal of molecular sciences (2024)
The role of adipose mesenchymal stem cells (Ad-MSCs) in metabolic syndrome remains unclear. We aimed to assess the expression of selected microRNAs in Ad-MSCs of non-diabetic adults in relation to Ad-MSC secretion of protein regulators and basic metabolic parameters. Ten obese, eight overweight, and five normal weight subjects were enrolled: 19 females and 4 males; aged 43.0 ± 8.9 years. Ad-MSCs were harvested from abdominal subcutaneous fat. Ad-MSC cellular expressions of four microRNAs (2 -ΔCt values) and concentrations of IL-6, IL-10, VEGF, and IGF-1 in the Ad-MSC-conditioned medium were assessed. The expressions of miR-21, miR-122, or miR-192 did not correlate with clinical parameters (age, sex, BMI, visceral fat, HOMA-IR, fasting glycemia, HbA1c, serum lipids, CRP, and eGFR). Conversely, the expression of miR-155 was lowest in obese subjects (3.69 ± 2.67 × 10 -3 vs. 7.07 ± 4.42 × 10 -3 in overweight and 10.25 ± 7.05 × 10 -3 in normal weight ones, p = 0.04). The expression of miR-155 correlated inversely with BMI (sex-adjusted r = -0.64; p < 0.01), visceral adiposity (r = -0.49; p = 0.03), and serum CRP (r = -0.63; p < 0.01), whereas it correlated positively with serum HDL cholesterol (r = 0.51; p = 0.02). Moreover, miR-155 synthesis was associated marginally negatively with Ad-MSC secretion of IGF-1 (r = -0.42; p = 0.05), and positively with that of IL-10 (r = 0.40; p = 0.06). Ad-MSC expression of miR-155 appears blunted in visceral obesity, which correlates with Ad-MSC IGF-1 hypersecretion and IL-10 hyposecretion, systemic microinflammation, and HDL dyslipidemia. Ad-MSC studies in metabolic syndrome should focus on miR-155.
Keyphrases
- long non coding rna
- metabolic syndrome
- poor prognosis
- insulin resistance
- cell proliferation
- adipose tissue
- mesenchymal stem cells
- long noncoding rna
- weight loss
- weight gain
- binding protein
- body mass index
- type diabetes
- high fat diet
- physical activity
- stem cells
- umbilical cord
- small cell lung cancer
- computed tomography
- endothelial cells
- magnetic resonance imaging
- cardiovascular risk factors
- skeletal muscle
- protein protein
- positron emission tomography
- magnetic resonance
- signaling pathway
- small molecule
- body weight
- pet ct
- obese patients
- glycemic control