Intracellular negative feedback mechanisms in blubber and muscle moderate acute stress responses in fasting seals.
Jessica G AvalosElizabeth R PiotrowskiAllison D NortheyDaniel E CrockerJane I KhudyakovPublished in: The Journal of experimental biology (2023)
Animals may limit the cost of stress responses during key life history stages such as breeding and molting by reducing tissue sensitivity to energy-mobilizing stress hormones (e.g., cortisol). We measured expression of genes encoding glucocorticoid receptor (GR, NR3C1), GR inhibitor (FKBP5), and cortisol-inactivating enzyme (HSD11B2) in blubber and muscle of northern elephant seals before and after stress axis stimulation by adrenocorticotropic hormone (ACTH) early and late in a fasting period associated with molting. ACTH elevated cortisol levels for >24 hours and increased FKBP5 and HSD11B2 expression while downregulating NR3C1 in blubber and muscle, suggesting robust intracellular negative feedback in peripheral tissues. This feedback was maintained over prolonged fasting, despite differences in baseline cortisol and gene expression levels between early and late molt, suggesting that fasting-adapted animals use tissue-specific, intracellular negative feedback mechanisms to modulate downstream impacts of acute stress responses during key life history stages.
Keyphrases
- blood glucose
- gene expression
- insulin resistance
- skeletal muscle
- poor prognosis
- liver failure
- respiratory failure
- reactive oxygen species
- drug induced
- aortic dissection
- blood pressure
- metabolic syndrome
- genome wide
- type diabetes
- long non coding rna
- high intensity
- stress induced
- hepatitis b virus
- extracorporeal membrane oxygenation
- heat stress
- glycemic control
- bioinformatics analysis
- genome wide analysis