Association of IS Vsa3 with Multidrug Resistance in Salmonella enterica Isolates from Cattle ( Bos taurus ).
Gentry L LewisRobert J FentonEtsuko N MoriyamaJohn Dustin LoyRodney A MoxleyPublished in: Microorganisms (2023)
Salmonella enterica is, globally, an important cause of human illness with beef being a significant attributable source. In the human patient, systemic Salmonella infection requires antibiotic therapy, and when strains are multidrug resistant (MDR), no effective treatment may be available. MDR in bacteria is often associated with the presence of mobile genetic elements (MGE) that mediate horizontal spread of antimicrobial resistance (AMR) genes. In this study, we sought to determine the potential relationship of MDR in bovine Salmonella isolates with MGE. The present study involved 111 bovine Salmonella isolates obtained collectively from specimens derived from healthy cattle or their environments at Midwestern U.S. feedyards (2000-2001, n = 19), or specimens from sick cattle submitted to the Nebraska Veterinary Diagnostic Center (2010-2020, n = 92). Phenotypically, 33/111 isolates (29.7%) were MDR (resistant to ≥3 drug classes). Based on whole-genome sequencing (WGS; n = 41) and PCR ( n = 111), a MDR phenotype was strongly associated (OR = 186; p < 0.0001) with carriage of IS Vsa3 , an IS 91 -like Family transposase. In all 41 isolates analyzed by WGS ((31 MDR and 10 non-MDR (resistant to 0-2 antibiotic classes)), MDR genes were associated with carriage of IS Vsa3 , most often on an IncC type plasmid carrying bla CMY-2 . The typical arrangement was floR , tet(A) , aph(6)-Id , aph(3″)-Ib , and sul2 flanked by IS Vsa3 . These results suggest that AMR genes in MDR S. enterica isolates of cattle are frequently associated with IS Vsa3 and carried on IncC plasmids. Further research is needed to better understand the role of IS Vsa3 in dissemination of MDR Salmonella strains.
Keyphrases
- multidrug resistant
- escherichia coli
- drug resistant
- klebsiella pneumoniae
- acinetobacter baumannii
- gram negative
- antimicrobial resistance
- genome wide
- endothelial cells
- genetic diversity
- listeria monocytogenes
- gene expression
- stem cells
- induced pluripotent stem cells
- case report
- climate change
- ultrasound guided
- bone marrow
- transcription factor
- pluripotent stem cells
- bioinformatics analysis
- genome wide identification
- electronic health record