CRISPR-mediated multigene integration enables Shikimate pathway refactoring for enhanced 2-phenylethanol biosynthesis in Kluyveromyces marxianus.

Mengwan LiXuye LangMarcos Moran CabreraSawyer De KeyserXiyan SunNancy Da SilvaIan Wheeldon

Published in: Biotechnology for biofuels (2021)

The CRISPR-mediated multigene integration system expands the genome-editing toolset for K. marxianus, a promising multi-stress tolerant host for the biosynthesis of 2-PE and other aromatic compounds derived from the Shikimate pathway.

Keyphrases

genome editing
crispr cas
cell wall
gene expression
stress induced
heat stress