Molecular Imaging of Biological Samples on Nanophotonic Laser Desorption Ionization Platforms.
Sylwia A StopkaCharles RongAndrew R KorteSridevi YadavilliJavad NazarianTrust T RazunguzwaNicholas J MorrisAkos VertesPublished in: Angewandte Chemie (International ed. in English) (2016)
Mass spectrometry imaging (MSI) is a comprehensive tool for the analysis of a wide range of biomolecules. The mainstream method for molecular MSI is matrix-assisted laser desorption ionization, however, the presence of a matrix results in spectral interferences and the suppression of some analyte ions. Herein we demonstrate a new matrix-free MSI technique using nanophotonic ionization based on laser desorption ionization (LDI) from a highly uniform silicon nanopost array (NAPA). In mouse brain and kidney tissue sections, the distributions of over 80 putatively annotated molecular species are determined with 40 μm spatial resolution. Furthermore, NAPA-LDI-MS is used to selectively analyze metabolites and lipids from sparsely distributed algal cells and the lamellipodia of human hepatocytes. Our results open the door for matrix-free MSI of tissue sections and small cell populations by nanophotonic ionization.
Keyphrases
- mass spectrometry
- high resolution
- gas chromatography
- ms ms
- induced apoptosis
- endothelial cells
- single molecule
- liquid chromatography
- multiple sclerosis
- minimally invasive
- cell cycle arrest
- magnetic resonance imaging
- high performance liquid chromatography
- high throughput
- oxidative stress
- capillary electrophoresis
- computed tomography
- stem cells
- signaling pathway
- fatty acid
- tandem mass spectrometry
- cell proliferation
- fluorescence imaging