Application of antibody phage display to identify potential antigenic neural precursor cell proteins.
Ioannis PaspaltsisEvangelia KesidouOlga TouloumiRoza LagoudakiMarina BozikiMartina SamiotakiDimitra DafouTheodoros SklaviadisNikolaos GrigoriadisPublished in: Journal of biological research (Thessalonike, Greece) (2020)
Using a standard immunization protocol to administer NPCs antigens, and applying the Fab fragment phage display technique, we were able to isolate at least a monoclonal IgG Fab fragment, which interacts with different mouse brain proteins. It is not clear whether such antibodies are produced in the host organisms, following NPCs transplantation.