Antibacterial effectiveness of trans-cinnamaldehyde against foodborne Enterobacteriaceae and its adjuvant effect with gentamicin.
Benise Ferreira da SilvaAntônio Mateus Gomes PereiraPaulo Adenes Teixeira CoelhoMarília Viana Albuquerque de AlmeidaHélcio Silva Dos SantosVictor Alves CarneiroRenata Albuquerque CostaPublished in: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2024)
The Enterobacteriaceae family is recognized as a primary group of Gram-negative pathogens responsible for foodborne illnesses and is frequently associated with antibiotic resistance. The present study explores the natural-based compound trans-cinnamaldehyde (TC) against drug-resistant Enterobacteriaceae and its synergism with gentamicin (GEN) to address this issue. The research employs three strains of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae, previously isolated from shrimp. The antibacterial activity was evaluated by the disk diffusion method, microdilution test, kinetics of growth, and time-kill curve. In addition, the synergistic effect between TC/GEN was investigated by checkerboard assay. All strains showed sensitivity to TC with an inhibition zone diameter > 35 mm. The TC showed inhibitory and bactericidal action in the most tested bacteria around 625 μg/mL. Sub-inhibitory amounts (1/2 and 1/4 MIC) of TC interfered with the growth kinetics by lag phase extension and decreased the log phase. Time-kill curves show a reduction of viable cells after the first hour of TC treatment at bactericidal concentrations. The synergistic effect between TC/GEN was observed for E. coli and E. cloacae strains with FICi ranging from 0.15 to 0.50. These findings, therefore, suggest TC as a promising alternative in the fight against drug-resistant Enterobacteriaceae that can cause foodborne illnesses.
Keyphrases
- multidrug resistant
- drug resistant
- gram negative
- klebsiella pneumoniae
- escherichia coli
- acinetobacter baumannii
- induced apoptosis
- pseudomonas aeruginosa
- randomized controlled trial
- blood pressure
- cystic fibrosis
- staphylococcus aureus
- atomic force microscopy
- endoplasmic reticulum stress
- antimicrobial resistance
- early stage
- cell cycle arrest
- signaling pathway
- wound healing