Characterization of different fat depots in NAFLD using inflammation-associated proteome, lipidome and metabolome.
Alen LovricMarit GranérElias BjornsonMuhammad ArifRui BenfeitasKristofer NymanMarcus StåhlmanMarkku O PentikäinenJesper LundbomAntti HakkarainenReijo SirénMarkku S NieminenNina LundbomKirsi LauermaMarja-Riitta TaskinenAdil MardinogluJan BorénPublished in: Scientific reports (2018)
Non-alcoholic fatty liver disease (NAFLD) is recognized as a liver manifestation of metabolic syndrome, accompanied with excessive fat accumulation in the liver and other vital organs. Ectopic fat accumulation was previously associated with negative effects at the systemic and local level in the human body. Thus, we aimed to identify and assess the predictive capability of novel potential metabolic biomarkers for ectopic fat depots in non-diabetic men with NAFLD, using the inflammation-associated proteome, lipidome and metabolome. Myocardial and hepatic triglycerides were measured with magnetic spectroscopy while function of left ventricle, pericardial and epicardial fat, subcutaneous and visceral adipose tissue were measured with magnetic resonance imaging. Measured ectopic fat depots were profiled and predicted using a Random Forest algorithm, and by estimating the Area Under the Receiver Operating Characteristic curves. We have identified distinct metabolic signatures of fat depots in the liver (TAG50:1, glutamate, diSM18:0 and CE20:3), pericardium (N-palmitoyl-sphinganine, HGF, diSM18:0, glutamate, and TNFSF14), epicardium (sphingomyelin, CE20:3, PC38:3 and TNFSF14), and myocardium (CE20:3, LAPTGF-β1, glutamate and glucose). Our analyses highlighted non-invasive biomarkers that accurately predict ectopic fat depots, and reflect their distinct metabolic signatures in subjects with NAFLD.
Keyphrases
- adipose tissue
- magnetic resonance imaging
- insulin resistance
- metabolic syndrome
- fatty acid
- oxidative stress
- high fat diet
- computed tomography
- type diabetes
- mass spectrometry
- heart failure
- physical activity
- high resolution
- left ventricular
- blood pressure
- cardiovascular disease
- pulmonary artery
- dna methylation
- weight gain
- magnetic resonance
- mitral valve