A 250-kDa glycoprotein of Naegleria fowleri induces protection and modifies the expression of α4β1 and LFA-1 on T and B lymphocytes in mouse meningitis model.
Diego A Castillo-RamírezMaría Maricela Carrasco-YépezItzel Berenice Rodríguez-MeraAldo Arturo Reséndiz-AlborÉrica Rosales-CruzRojas-Hernández SaúlPublished in: Parasite immunology (2021)
The aims of this work were to evaluate the protective role of the 250-kDa polypeptide band of Naegleria fowleri. We designed an immunization strategy in Balb/c mice which were inoculated by i.n. route with an electrocuted 250-kDa polypeptide band of N. fowleri. We observed that the 250-kDa band induced 80% of protection, whereas the coadministration with Cholera Toxin induced 100% of protection. Moreover, high levels of IgA- and IgG-specific antibodies were detected by ELISA assay. We also analysed migration molecules (α4β1 and LFA-1) on T and B lymphocytes in nose-associated lymphoid tissue (NALT), cervical lymph nodes (CN) and nasal passages (NP) by flow cytometry. We observed that the percentage of B cells (B220/α4β1) and T cells (CD4/α4β1) in NP were higher in all immunized groups compared with the other compartments analysed. Finally, we detected by immunohistochemistry ICAM-1 and V-CAM-1 in the nasal cavity. The immunization with the 250-kDa polypeptide band, protect mice against N. fowleri challenge and modifies migration molecules and their ligands.
Keyphrases
- heat shock protein
- flow cytometry
- lymph node
- high glucose
- diabetic rats
- peripheral blood
- high fat diet induced
- poor prognosis
- escherichia coli
- drug induced
- chronic rhinosinusitis
- endothelial cells
- oxidative stress
- lymph node metastasis
- skeletal muscle
- metabolic syndrome
- sentinel lymph node
- neoadjuvant chemotherapy
- stress induced