Serum Phthalate Concentrations and Biomarkers of Oxidative Stress in Adipose Tissue in a Spanish Adult Cohort.
Celia Pérez-DíazFrancisco M Pérez-CarrascosaBlanca Riquelme-GallegoElena Villegas-AranaAlejandro Joaquín ArmendarizJavier Galindo-ÁngelHanne FrederiksenJosefa LeónPilar RequenaJuan Pedro ArrebolaPublished in: Environmental science & technology (2024)
The relationship between phthalates, a group of chemical pollutants classified as endocrine disruptors, and oxidative stress is not fully understood. The aim of the present hospital-based study was to explore the associations between circulating levels of 10 phthalate metabolites and 8 biomarkers of oxidative stress in adipose tissue. The study population ( n = 143) was recruited in two hospitals in the province of Granada (Spain). Phthalate metabolite concentrations were analyzed by isotope diluted online-TurboFlow-LC-MS/MS in serum samples, while oxidative stress markers were measured by commercially available kits in adipose tissue collected during routine surgery. Statistical analyses were performed by MM estimators' robust linear regression and weighted quantile sum regression. Mainly, positive associations were observed of monomethyl phthalate (MMP), monoiso-butyl phthalate (MiBP), and mono- n -butyl phthalate (MnBP) (all low molecular weight phthalates) with glutathione peroxidase (GPx) and thiobarbituric acid reactive substances (TBARS), while an inverse association was found between monoiso-nonyl phthalate (MiNP) (high molecular weight phthalate) and the same biomarkers. WQS analyses showed significant effects of the phthalate mixture on GSH (β = -30.089; p -value = 0.025) and GSSG levels (β = -19.591; p -value = 0.030). Despite the limitations inherent to the cross-sectional design, our novel study underlines the potential influence of phthalate exposure on redox homeostasis, which warrants confirmation in further research.
Keyphrases
- oxidative stress
- adipose tissue
- cross sectional
- insulin resistance
- high fat diet
- emergency department
- diabetic rats
- young adults
- minimally invasive
- mass spectrometry
- clinical practice
- acute coronary syndrome
- climate change
- induced apoptosis
- percutaneous coronary intervention
- endoplasmic reticulum stress
- contrast enhanced
- fluorescent probe
- childhood cancer