An intrinsically semi-permeable PDMS nanosheet encapsulating adipose tissue-derived stem cells for enhanced angiogenesis.
Megumi TakumaHajime FujitaNanami ZushiHisato NaganoRyuichi AzumaTomoharu KiyosawaToshinori FujiePublished in: Biomaterials science (2024)
Cell encapsulation devices are expected to be promising tools that can control the release of therapeutic proteins secreted from transplanted cells. The protein permeability of the device membrane is important because it allows the isolation of transplanted cells while enabling the effectiveness of the device. In this study, we investigated free-standing polymeric ultra-thin films (nanosheets) as an intrinsically semi-permeable membrane made from polydimethylsiloxane (PDMS). The PDMS nanosheet with a thickness of 600 nm showed intrinsic protein permeability, and the device fabricated with the PDMS nanosheet showed that VEGF secreted from implanted adipose tissue-derived stem cells (ASCs) could be released for at least 5 days. The ASC encapsulation device promoted angiogenesis and the development of granulation tissue 1 week after transplantation to the subcutaneous area of a mouse. This cell encapsulation device consisting of PDMS nanosheets provides a new method for pre-vascularization of the subcutaneous area in cell transplantation therapy.
Keyphrases
- stem cells
- cell therapy
- adipose tissue
- endothelial cells
- induced apoptosis
- single cell
- vascular endothelial growth factor
- randomized controlled trial
- insulin resistance
- systematic review
- drug delivery
- cell cycle arrest
- high fat diet
- quantum dots
- high resolution
- photodynamic therapy
- gold nanoparticles
- endoplasmic reticulum stress
- metabolic syndrome
- binding protein
- oxidative stress
- reduced graphene oxide
- cell death
- highly efficient
- nlrp inflammasome
- clinical trial
- cell proliferation
- small molecule
- protein protein
- skeletal muscle
- pi k akt
- atomic force microscopy