Methods in enzymology

Keyphrases

Publications

volume 704, 2024

Peter J Harrison, Jake Chandler, Andrew J Thompson, Timothy D H Bugg
In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays. Methods in enzymology 704 (2024)
Paul F Fitzpatrick, S Colette Daubner
Biochemical and biophysical approaches to characterization of the aromatic amino acid hydroxylases. Methods in enzymology 704 (2024)
Tzu-Yu Chen, Lide Cha, Jared C Paris, Yisong Guo, Wei-Chen Chang
Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxoglutarate dependent enzymes. Methods in enzymology 704 (2024)
Jai Krishna Mahto, Arpan Kayastha, Pravindra Kumar
Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: Biphenyl, Phthalate, and Terephthalate dioxygenases. Methods in enzymology 704 (2024)
Melanie S Rogers, John D Lipscomb
Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase. Methods in enzymology 704 (2024)
Hui Tao, Ikuro Abe
Functional analysis of an α-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis. Methods in enzymology 704 (2024)
Ramaswamy Subramanian
Methods used to determine the structure of the oxygenase component of naphthalene 1,2 dioxygenase. Methods in enzymology 704 (2024)
Daan Ren, Yu-Hsuan Lee, Hung-Wen Liu
Expression, purification and characterization of non-heme iron-dependent mono-oxygenase OzmD in oxazinomycin biosynthesis. Methods in enzymology 704 (2024)
Andrew Jakobowski, S Gage Hill, S Wyatt Guy, Adam R Offenbacher
Substitution of the mononuclear, non-heme iron cofactor in lipoxygenases for structural studies. Methods in enzymology 704 (2024)
Austin R Hopiavuori, Radcliff T Huffman, Shaun M K McKinnie
Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases. Methods in enzymology 704 (2024)
Lennart Brewitz, Amelia Brasnett, Lara I Schnaubelt, Patrick Rabe, Anthony Tumber, Christopher J Schofield
Methods for production and assaying catalysis of isolated recombinant human aspartate/asparagine-β-hydroxylase. Methods in enzymology 704 (2024)
Antonio Del Rio Flores, Rui Zhai, Wenjun Zhang
Isonitrile biosynthesis by non-heme iron(II)-dependent oxidases/decarboxylases. Methods in enzymology 704 (2024)
Jackson Campbell, Yifan Wang
Observing extradiol dioxygenases in action through a crystalline lens. Methods in enzymology 704 (2024)
Shan Xue, Yijie Tang, Igor V Kurnikov, Hsuan-Jen Liao, Jikun Li, Nei-Li Chan, Maria G Kurnikova, Wei-Chen Chang, Yisong Guo
Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJ. Methods in enzymology 704 (2024)

volume 703, 2024

Charlotte E Bopp, Nora M Bernet, Sarah G Pati, Thomas B Hofstetter
Characterization of O 2 uncoupling in biodegradation reactions of nitroaromatic contaminants catalyzed by rieske oxygenases. Methods in enzymology 703 (2024)
Jessica Lusty Beech, Julia Ann Fecko, Neela Yennawar, Jennifer L DuBois
Functional and spectroscopic approaches to determining thermal limitations of Rieske oxygenases. Methods in enzymology 703 (2024)
Zachary D Bennett, Thomas C Brunold
Non-standard amino acid incorporation into thiol dioxygenases. Methods in enzymology 703 (2024)
Edward I Solomon, Robert R Gipson
Spectroscopic definition of ferrous active sites in non-heme iron enzymes. Methods in enzymology 703 (2024)
Megan E Wolf, Lindsay D Eltis
Preparation of reductases for multicomponent oxygenases. Methods in enzymology 703 (2024)
Clara Graves, Kabirul Islam
Development of a rapid mass spectrometric method for the analysis of ten-eleven translocation enzymes. Methods in enzymology 703 (2024)
Ran Duan, Jiasong Li, Aimin Liu
Unveiling the mechanism of cysteamine dioxygenase: A combined HPLC-MS assay and metal-substitution approach. Methods in enzymology 703 (2024)
Elizabeth R Smithwick, Ambika Bhagi-Damodaran, Anoop Rama Damodaran
Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase. Methods in enzymology 703 (2024)
Tao Zhang, Kelin Li, Yuk Hei Cheung, Mark W Grinstaff, Pinghua Liu
Photo-reduction facilitated stachydrine oxidative N-demethylation reaction: A case study of Rieske non-heme iron oxygenase Stc2 from Sinorhizobium meliloti. Methods in enzymology 703 (2024)
Gage T Barroso, Alejandro Arcadio Garcia, Madison Knapp, David G Boggs, Jennifer Bridwell-Rabb
Purification and characterization of a Rieske oxygenase and its NADH-regenerating partner proteins. Methods in enzymology 703 (2024)
Qun Zhao, Jinyan Rui, Xiongyi Huang
Radical-relay C(sp 3 )-H azidation catalyzed by an engineered nonheme iron enzyme. Methods in enzymology 703 (2024)
Niels A W de Kok, Hui Miao, Sandy Schmidt
In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01. Methods in enzymology 703 (2024)
Meredith B Mock, Shuyuan Zhang, Ryan M Summers
Whole-cell Rieske non-heme iron biocatalysts. Methods in enzymology 703 (2024)

volume 702, 2024

Prosenjit Ray, Chelsea R Rand-Fleming, Steven O Mansoorabadi
Preparation of coenzyme F430 biosynthetic enzymes and intermediates. Methods in enzymology 702 (2024)
Reyvin M Reyes, Amy C Rosenzweig
Purification and biochemical characterization of methanobactin biosynthetic enzymes. Methods in enzymology 702 (2024)
Noah S Lyons, Sydney B Johnson, Pablo Sobrado
Methods for biochemical characterization of flavin-dependent N-monooxygenases involved in siderophore biosynthesis. Methods in enzymology 702 (2024)
Tomas Richardson-Sanchez, Thomas J Telfer, Cho Z Soe, Kate P Nolan, Michael P Gotsbacher, Rachel Codd
The production of siderophore analogues using precursor-directed biosynthesis. Methods in enzymology 702 (2024)
Andrew M Gulick, Lisa S Mydy, Ketan D Patel
Kinetic analysis of the three-substrate reaction mechanism of an NRPS-independent siderophore (NIS) synthetase. Methods in enzymology 702 (2024)
Melanie Susman, Jin Yan, Christina Makris, Alison Butler
Discovery, isolation, and characterization of diazeniumdiolate siderophores. Methods in enzymology 702 (2024)
Minuri Ratnayake, Y T Candace Ho, Xinyun Jian, Max J Cryle
An in vitro assay to explore condensation domain specificity from non-ribosomal peptide synthesis. Methods in enzymology 702 (2024)
Rachel N Motz, Ghazal Kamyabi, Elizabeth M Nolan
Experimental methods for evaluating siderophore-antibiotic conjugates. Methods in enzymology 702 (2024)
Thiago M Pasin, Kathleen M Meneely, Deegan M Ruiz, Audrey L Lamb
A continuous fluorescence assay to measure nicotianamine synthase activity. Methods in enzymology 702 (2024)
Marquis T Yazzie, Zachary L Reitz, Robin Schmid, Daniel Petras, Allegra T Aron
Native metabolomics for mass spectrometry-based siderophore discovery. Methods in enzymology 702 (2024)
Andrew M Gulick, Lisa S Mydy, Ketan D Patel
Kinetic analysis of the three-substrate reaction mechanism of an NRPS-independent siderophore (NIS) synthetase. Methods in enzymology 702 (2024)
Zachary L Reitz
Predicting metallophore structure and function through genome mining. Methods in enzymology 702 (2024)
C E Merrick, N M Gulati, T A Wencewicz
Siderophore-dependent ferrichelatases. Methods in enzymology 702 (2024)
Vivian Kitainda, Joseph Jez
4-Aldrithiol-based photometric assay for detection of methylthioalkylmalate synthase activity. Methods in enzymology 702 (2024)
Ronivaldo Rodrigues da Silva, Arnab Kumar Nath, Victoria Adedoyin, Emmanuel Akpoto, Jennifer L DuBois
Anaerobic heme recycling by gut microbes: Important methods for monitoring porphyrin production. Methods in enzymology 702 (2024)
Katherine M Hoffmann, Jocelin D Hernandez, Eliana G Goncuian, Nathan L March
ITC-based kinetics assay for NIS synthetases. Methods in enzymology 702 (2024)
Tashi C E Liebergesell, Aaron W Puri
Linking biosynthetic genes to natural products using inverse stable isotopic labeling (InverSIL). Methods in enzymology 702 (2024)

volume 701, 2024

Soohyung Park, Richard W Pastor, Wonpil Im
Binary bilayer simulations for partitioning within membranes. Methods in enzymology 701 (2024)
Jessica Bodosa, Anthony J Pane, Jeffery B Klauda
Modeling asymmetric cell membranes at all-atom resolution. Methods in enzymology 701 (2024)
Marius F W Trollmann, Rainer A Böckmann
Characterization of domain formation in complex membranes. Methods in enzymology 701 (2024)
Kshitiz Parihar, Seung-Hyun Ko, Ryan Bradley, Phillip Taylor, N Ramakrishnan, Tobias Baumgart, Wei Guo, Valerie M Weaver, Paul A Janmey, Ravi Radhakrishnan
Free energy calculations for membrane morphological transformations and insights to physical biology and oncology. Methods in enzymology 701 (2024)
Tugba Nur Ozturk, Melanie König, Timothy S Carpenter, Kasper B Pedersen, Tsjerk A Wassenaar, Helgi I Ingólfsson, Siewert J Marrink
Building complex membranes with Martini 3. Methods in enzymology 701 (2024)
Long Li, Jie Gao, Łukasz Milewski, Jinglei Hu, Bartosz Różycki
Lattice-based mesoscale simulations and mean-field theory of cell membrane adhesion. Methods in enzymology 701 (2024)