Diagnostic Efficacy of LAMP Assay for Human Fungal Pathogens: a Systematic Review and Meta-analysis.
Gurvinder Singh BumbrahSarika JainShweta SinghZeeshan FatimaSaif HameedPublished in: Current fungal infection reports (2023)
From various studies reported on the diagnosis of fungi, only 9 articles were identified as eligible to meet the criteria of LAMP based diagnosis. Through this meta-analysis, it was found that most of the studies were conducted in China and Japan with sputum and blood as the most common specimens to be used for LAMP assay. The collected data underlined that ITS gene and fluorescence-based detections ranked as the most used target and method. The pooled sensitivity values of meta-analysis ranged between 0.71 and 1.0 and forest plot and SROC (summary receiver operating characteristic) curve revealed a pooled specificity values between 0.13 and 1.0 with the confidence interval of 95%, respectively. The accuracy and precision rates of eligible studies mostly varied between 70 to 100% and 68 to 100%, respectively. A quality assessment based on QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies) of bias and applicability was conducted which depicted low risk of bias and applicability concerns. Together, LAMP technology could be considered as a feasible alternative to current diagnostics considering high fungal burden for rapid testing in low resource regions.
Keyphrases
- case control
- loop mediated isothermal amplification
- systematic review
- high throughput
- endothelial cells
- meta analyses
- sensitive detection
- genome wide
- dna methylation
- randomized controlled trial
- clinical trial
- gene expression
- copy number
- big data
- pulmonary tuberculosis
- gram negative
- artificial intelligence
- double blind
- fine needle aspiration
- genome wide identification
- pluripotent stem cells