A binary interaction map between turnip mosaic virus and Arabidopsis thaliana proteomes.
Fernando MartínezJosé L CarrascoChristina ToftJulia HillungSilvia Giménez-SantamarinaLynne YenushGuillermo RodrigoSantiago F ElenaPublished in: Communications biology (2023)
Viruses are obligate intracellular parasites that have co-evolved with their hosts to establish an intricate network of protein-protein interactions. Here, we followed a high-throughput yeast two-hybrid screening to identify 378 novel protein-protein interactions between turnip mosaic virus (TuMV) and its natural host Arabidopsis thaliana. We identified the RNA-dependent RNA polymerase NIb as the viral protein with the largest number of contacts, including key salicylic acid-dependent transcription regulators. We verified a subset of 25 interactions in planta by bimolecular fluorescence complementation assays. We then constructed and analyzed a network comprising 399 TuMV-A. thaliana interactions together with intravirus and intrahost connections. In particular, we found that the host proteins targeted by TuMV are enriched in different aspects of plant responses to infections, are more connected and have an increased capacity to spread information throughout the cell proteome, display higher expression levels, and have been subject to stronger purifying selection than expected by chance. The proviral or antiviral role of ten host proteins was validated by characterizing the infection dynamics in the corresponding mutant plants, supporting a proviral role for the transcriptional regulator TGA1. Comparison with similar studies with animal viruses, highlights shared fundamental features in their mode of action.
Keyphrases
- arabidopsis thaliana
- protein protein
- high throughput
- transcription factor
- small molecule
- single cell
- poor prognosis
- gene expression
- cell therapy
- cancer therapy
- wastewater treatment
- single molecule
- healthcare
- stem cells
- case control
- reactive oxygen species
- network analysis
- drug delivery
- health information
- long non coding rna
- social media
- genetic diversity
- finite element
- wild type
- energy transfer
- plasmodium falciparum