α -Glucosidase Inhibitory Activity and Anti-Adipogenic Effect of Compounds from Dendrobium delacourii .
May Thazin ThantHnin Ei Ei KhineJustin Quiel Lasam NealigaNutputsorn ChatsumpunChatchai ChaothamBoonchoo SritularakKittisak LikhitwitayawuidPublished in: Molecules (Basel, Switzerland) (2022)
Chemical investigation of Dendrobium delacourii revealed 11 phenolic compounds, and the structures of these compounds were determined by analysis of their NMR and HR-ESI-MS data. All compounds were investigated for their α -glucosidase inhibitory activity and anti-adipogenic properties. Phoyunnanin E ( 10 ) and phoyunnanin C ( 11 ) showed the most potent α -glucosidase inhibition by comparing with acarbose, which was used as a positive control. Kinetic study revealed the non-competitive inhibitors against the enzyme. For anti-adipogenic activity, densifloral B ( 3 ) showed the strongest inhibition when compared with oxyresveratrol (positive control). In addition, densifloral B might be responsible for the inhibition of adipocyte differentiation via downregulating the expression of peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT enhancer-binding protein alpha (C/EBP α ), which are major transcription factors in adipogenesis.
Keyphrases
- binding protein
- molecular docking
- transcription factor
- ms ms
- high resolution
- insulin resistance
- single cell
- mass spectrometry
- adipose tissue
- poor prognosis
- magnetic resonance
- fatty acid
- multiple sclerosis
- type diabetes
- big data
- atomic force microscopy
- solid state
- molecular dynamics simulations
- genome wide identification