Mycotoxins produced by Aspergillus spp., Penicillium spp. and Fusarium spp. with small molecular weight and thermal stability, are highly toxic and carcinogenic secondary metabolites. Mycotoxins have caused widespread concern regarding food safety internationally because of their adverse effects on the health of humans and animals, and the major economic losses they cause. There is an urgent need to find ways to reduce or eliminate the impact of mycotoxins in food and feed without introducing new safety issues, or reducing nutritional quality. Non-thermal physical technology is the basis for new techniques to degrade mycotoxins, with great potential for practical detoxification applications in the food industry. Compared with conventional thermal treatments, non-thermal physical detoxification technologies are easier to apply and effective, with less adverse impact on the nutritional value of agricultural products. The advantages, limitations and development prospects of these new detoxification technologies are discussed. Further studies are recommended to standardize the treatment conditions for each detoxification technology, evaluate the safety of the degradation products, and to combine different detoxification technologies to achieve synergistic effects. This will facilitate realization of the great potential of the new technologies and the development of practical applications.