mTORC1 Mediates Biphasic Mechano-Response to Orchestrate Adhesion-Dependent Cell Growth and Anoikis Resistance.
Chunlei ZhangYuan WangZifeng ZhenJiayi LiJing SuCongying WuPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2023)
Cells constantly sense and respond to not only biochemical but also biomechanical changes in their microenvironment, demanding for dynamic metabolic adaptation. ECM stiffening is a hallmark of cancer aggressiveness, while survival under substrate detachment also associates with poor prognosis. Mechanisms underlying this, non-linear mechano-response of tumor cells may reveal potential double-hit targets for cancers. Here, an integrin-GSK3β-FTO-mTOR axis is reported, that can integrate stiffness sensing to ensure both the growth advantage endowed by rigid substrate and cell death resistance under matrix detachment. It is demonstrated that substrate stiffening can activate mTORC1 and elevate mTOR level through integrins and GSK3β-FTO mediated mRNA m 6 A modification, promoting anabolic metabolism. Inhibition of this axis upon ECM detachment enhances autophagy, which in turn conveys resilience of tumor cells to anoikis, as it is demonstrated in human breast ductal carcinoma in situ (DCIS) and mice malignant ascites. Collectively, these results highlight the biphasic mechano-regulation of cellular metabolism, with implications in tumor growth under stiffened conditions such as fibrosis, as well as in anoikis-resistance during cancer metastasis.
Keyphrases
- poor prognosis
- cell death
- papillary thyroid
- cell cycle arrest
- signaling pathway
- long non coding rna
- induced apoptosis
- pi k akt
- squamous cell
- endothelial cells
- cell proliferation
- stem cells
- endoplasmic reticulum stress
- climate change
- genome wide
- childhood cancer
- amino acid
- single cell
- type diabetes
- dna methylation
- lymph node metastasis
- gene expression
- adipose tissue
- depressive symptoms
- young adults
- cystic fibrosis
- sensitive detection
- cell adhesion
- pluripotent stem cells
- insulin resistance
- wild type