Maternal sucralose exposure induces Paneth cell defects and exacerbates gut dysbiosis of progeny mice.
Xin DaiChen WangZixuan GuoYun LiTianyu LiuGe JinSinan WangBangmao WangKui JiangHailong CaoPublished in: Food & function (2021)
Research has shown that maternal sucralose (MS) exposure alters the gut microbiota of offspring at weaning and predisposes the offspring to developing obesity, non-alcoholic fatty liver disease and metabolic syndrome later in life. However, the underlying mechanism remains unclear. Paneth cells are thought to critically influence the gut microbiota. This study aimed to investigate whether MS exposure induced Paneth cell defects and exacerbated gut dysbiosis of offspring. Female C57BL/6 mice were divided into the MS and control (water) groups during pregnancy and lactation. Progeny mice were fed a normal sucralose-free diet after weaning until adulthood. MS inhibited intestinal development and increased the expression of proinflammatory cytokines in the small intestines of 3-week-old progeny mice. MS increased the proportions of abnormal granule secretion by Paneth cells. The number of Paneth cells and mRNA expression of AMPs such as cryptdins and lysozyme were reduced in the MS group. MS disturbed the gut microbiota composition and diversity in the 3-week-old offspring mice. The relative abundances of pro-inflammatory bacteria, such as Desulfovibrionales, Helicobacter, Pasteurellales and Campylobacterales were significantly increased in the MS group, while anti-inflammatory bacteria, including Clostridium XI, were decreased. This dysbiosis continued into adulthood. These findings showed that MS exposure induced Paneth cell defects and exacerbated gut dysbiosis in offspring mice. Sucralose should be consumed with caution, especially during pregnancy and in early life.
Keyphrases
- mass spectrometry
- multiple sclerosis
- ms ms
- high fat diet induced
- metabolic syndrome
- induced apoptosis
- early life
- insulin resistance
- high fat diet
- single cell
- cell cycle arrest
- type diabetes
- depressive symptoms
- randomized controlled trial
- anti inflammatory
- weight loss
- poor prognosis
- cell therapy
- pregnant women
- diabetic rats
- birth weight
- physical activity
- intensive care unit
- oxidative stress
- mesenchymal stem cells
- cell death
- extracorporeal membrane oxygenation
- adipose tissue
- drug induced
- human milk
- stress induced