Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression.

Audrey MasiKlara WögerbauerRobert L MachAstrid R Mach-Aigner

Published in: Fungal biology and biotechnology (2024)

The CRISPR/Cas9 transformation system presented in this study provides new opportunities for strain engineering of A. pullulans. This system allows expression of Cas9 and antibiotic resistance while being easy to adapt. This strategy could open the path to intensive genomic engineering in A. pullulans.

Keyphrases

crispr cas
genome editing
poor prognosis
copy number
minimally invasive
long non coding rna
escherichia coli
gene expression
genome wide