Simvastatin Attenuates Areca Nut Extract-Induced Subdermal Fibrosis in Mice by Targeting TGF-β Signaling Pathways.
Chi-Hua ChangChing-Ping LinYuk-Kwan ChenYu-Fang HsiaoYan-Hsiung WangPublished in: Current issues in molecular biology (2023)
Oral submucous fibrosis (OSMF) is a chronic inflammatory disease and a potentially malignant oral disorder, characterized by fibrosis of the oral mucosa. TGF-β signaling pathways have been implicated in the development of OSMF, with areca nut extract (ANE) contributing to the disease progression. Simvastatin, a statin drug, has demonstrated anti-fibrotic properties in various fibrotic conditions. However, its therapeutic potential in treating OSMF remains unclear. In this study, 8-week-old male BALB/c mice were randomly divided into three groups based on different time points. Each mouse was then treated with four different drug formulations. Post-treatment, specimens were collected for histopathological examination and staining to assess skin thickness, fibrosis, and collagen deposition. ANE treatment alone significantly increased skin thickness and collagen deposition compared to the control group after the 4-week time point. The combined administration of ANE and simvastatin, resulted in a notable reduction in skin thickness and collagen deposition. Western blot analysis revealed that simvastatin effectively suppressed the expression of fibrosis-related proteins, including CTGF, and α-SMA, in ANE-induced subdermal fibrosis. These results suggest that simvastatin has potential therapeutic effects on ANE-induced subdermal fibrosis, providing a foundation for future studies and possible clinical applications.
Keyphrases
- drug induced
- wound healing
- diabetic rats
- high glucose
- signaling pathway
- oxidative stress
- liver fibrosis
- poor prognosis
- soft tissue
- randomized controlled trial
- coronary artery disease
- transforming growth factor
- epithelial mesenchymal transition
- insulin resistance
- anti inflammatory
- metabolic syndrome
- single cell
- wild type
- flow cytometry
- double blind
- replacement therapy
- endoplasmic reticulum stress
- smoking cessation