Predicting the Pro-Inflammatory Effects of Oxidized Methyl Oleate Based on the Volatile Compounds.
Luocheng ZhangXinxin JiaoJie XiangSasa ZhaoJingyi WangJunsong XiaoHua WuPublished in: Journal of oleo science (2024)
The negative impact of lipid peroxidation on health is intimately tied to its oxidation products. In this study, methyl oleate was oxidized at 180℃ for 0, 2, 4, 8 and 12 h respectively. The free radicals and volatile components generated during the oxidation process were determined using electron spin resonance and headspace solid-phase microextraction (HS-SPME)-GC-MS. The pro-inflammatory effects of oxidized methyl oleate were evaluated in RAW264.7 cells. Then partial least-squares regression (PLSR) models were established for predicting the 3 pro-inflammatory genes expression based on the volatile components. The results revealed that the alkoxy radical content increased rapidly during oxidation from 4 h to 8 h, and the rate of oxidation of methyl oleate dropped after 8 h. A total of 27 volatile oxidation compounds were detected by HS-SPME-GC-MS. The content of most compounds, including aldehydes, esters, and acids, exhibited a pattern of initial increase and then decrease as the oxidation time increased. Similarly, the proinflammatory effects of oxidized methyl oleate peaked after 8 h of oxidation. The PLSR quantitative prediction models showed that the coefficient of determination (R 2 P ) between the predicted and measured values of the 3 inflammatory gene expressions were 0.915, 0.946 and 0.951 respectively. The established PLSR model predicts the pro-inflammatory effects of oxidized methyl oleate well and provides a theoretical foundation for quick evaluation of the pro-inflammatory effects of oxidized lipids.
Keyphrases
- hydrogen peroxide
- low density lipoprotein
- gas chromatography
- electron transfer
- healthcare
- visible light
- genome wide
- mental health
- nitric oxide
- oxidative stress
- fatty acid
- induced apoptosis
- magnetic resonance
- high resolution
- room temperature
- mass spectrometry
- social media
- long non coding rna
- copy number
- cell death
- dna methylation
- genome wide identification
- gene expression
- endoplasmic reticulum stress
- binding protein
- solid phase extraction
- bioinformatics analysis
- health promotion