Heimao tea polysaccharides ameliorate obesity by enhancing gut microbiota-dependent adipocytes thermogenesis in mice fed with high fat diet.

Heimao tea (HMT) is a kind of fermented dark tea that has various health benefits. However, the available information regarding the anti-obesity effect of HMT and its active ingredients is still limited. Herein, we extracted the polysaccharides from Heimao tea (HMTP) and evaluated the anti-obesity effect and the underlying mechanism of HMTP. 12-Week administration of HMTP ameliorated lipid accumulation in the adipose tissue and improved glucolipid metabolism in high-fat diet (HFD)-fed mice. HMTP also induced browning of inguinal white adipose tissue (iWAT) and enhanced the thermogenic activity of interscapular brown adipose tissue (iBAT) by upregulating the expression of a series of thermogenic genes, such as Ucp1, Prdm16, and Pgc1α. Interestingly, the anti-obesity effect of HMTP was closely associated with altered relative abundance of the gut microbes, especially Dubosiella and Romboutsia , with significant increases, in which the abundance of Dubosiella and Romboutsia was negatively correlated with the body weight ( r = -0.567, p < 0.05; r = -0.407, p < 0.05) and positively correlated with the iBAT index ( r = 0.520, p < 0.05; r = 0.315, p < 0.05). Our data suggest that the alteration of the gut microbiota may play a critical role in HMTP-induced iWAT browning and iBAT activation, and our findings may provide a promising way for preventing obesity.