CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2.
Yiren CaoJinjun WuJuan LiHongquan ZhangX Chris LePublished in: Chemical communications (Cambridge, England) (2021)
The trans-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a resulted in visual detection of the N gene and E gene of SARS-CoV-2 in 45 min.
Keyphrases
- crispr cas
- sars cov
- gold nanoparticles
- genome editing
- label free
- nucleic acid
- respiratory syndrome coronavirus
- loop mediated isothermal amplification
- genome wide
- copy number
- real time pcr
- reduced graphene oxide
- genome wide identification
- single molecule
- sensitive detection
- gene expression
- dna methylation
- living cells
- fluorescent probe
- genome wide analysis
- quantum dots