Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase.

Tom BenekeUlrich DobramyslCarolina Moura Costa Catta-PretaJeremy Charles MottramEva GluenzRichard John Wheeler

Published in: Wellcome open research (2022)

We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.

Keyphrases

genome editing
crispr cas
genome wide
copy number
dna methylation
molecularly imprinted
rna seq
gene expression
genome wide identification
transcription factor