Characterization of antibiotic resistance genes in the species of the rumen microbiota.
Yasmin Neves Vieira SabinoMateus Ferreira SantanaLinda Boniface OyamaFernanda Godoy SantosAna Júlia Silva MoreiraSharon Ann HuwsHilário Cuquetto MantovaniePublished in: Nature communications (2019)
Infections caused by multidrug resistant bacteria represent a therapeutic challenge both in clinical settings and in livestock production, but the prevalence of antibiotic resistance genes among the species of bacteria that colonize the gastrointestinal tract of ruminants is not well characterized. Here, we investigate the resistome of 435 ruminal microbial genomes in silico and confirm representative phenotypes in vitro. We find a high abundance of genes encoding tetracycline resistance and evidence that the tet(W) gene is under positive selective pressure. Our findings reveal that tet(W) is located in a novel integrative and conjugative element in several ruminal bacterial genomes. Analyses of rumen microbial metatranscriptomes confirm the expression of the most abundant antibiotic resistance genes. Our data provide insight into antibiotic resistange gene profiles of the main species of ruminal bacteria and reveal the potential role of mobile genetic elements in shaping the resistome of the rumen microbiome, with implications for human and animal health.
Keyphrases
- antibiotic resistance genes
- microbial community
- genome wide
- wastewater treatment
- copy number
- dna methylation
- multidrug resistant
- anaerobic digestion
- genome wide identification
- healthcare
- endothelial cells
- public health
- poor prognosis
- genetic diversity
- risk factors
- single cell
- mental health
- cystic fibrosis
- gene expression
- gram negative
- human health
- health information
- risk assessment
- escherichia coli
- pseudomonas aeruginosa
- long non coding rna
- climate change
- pluripotent stem cells