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Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize.

Yuan-Yuan JiangYi-Ping ChaiMin-Hui LuXiu-Li HanQiupeng LinYu ZhangQiang ZhangYun ZhouXue-Chen WangCaixia GaoQi-Jun Chen
Published in: Genome biology (2020)
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
Keyphrases
  • crispr cas
  • genome editing
  • poor prognosis
  • emergency department
  • gene expression
  • transcription factor
  • dna methylation
  • adverse drug
  • genome wide identification