Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media.
Se Hwan HwangWeonSun LeeSang Hi ParkHee Jin LeeSun Hwa ParkDong Chang LeeMi Hyun LimSang A BackByeong Gon YunJung Ho JeunJung Yeon LimJun Myung KangSung Won KimPublished in: PloS one (2017)
We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM. In addition, upregulation of IL-8 and GM-CSF and downregulation of RANTES expression were shown in response to SFXFM. Moreover, two-lineage differentiation properties (osteocyte and adipocyte) of hTMSCs were enhanced under SFXFM. Finally, the genetic stability of SFXFM-cultured hTMSCs was demonstrated by normal karyotype results. SFXFM enables good expansion, multipotentiality, and normal genotype maintenance of MSCs. Moreover, this approach serves as a substitute to conventional media for the cultivation of capable MSCs for upcoming medical applications.
Keyphrases
- endothelial cells
- mesenchymal stem cells
- stem cells
- signaling pathway
- poor prognosis
- end stage renal disease
- newly diagnosed
- umbilical cord
- cell proliferation
- healthcare
- ejection fraction
- single cell
- peritoneal dialysis
- prognostic factors
- cell therapy
- long non coding rna
- fatty acid
- binding protein
- insulin resistance
- dna methylation