Quantitative glycoproteomics of high-density lipoproteins.
Xinyu TangMaurice WongJennyfer TenaChenghao ZhuChristopher RhodesQingwen ZhouAnita VinjamuriArmin OloumiSucharita BodduGuillaume LuxardiEmanual MaverakisCarlito B LebrillaAngela M ZivkovicPublished in: RSC advances (2022)
In this work, we developed a targeted glycoproteomic method to monitor the site-specific glycoprofiles and quantities of the most abundant HDL-associated proteins using Orbitrap LC-MS for (glyco)peptide target discovery and QqQ LC-MS for quantitative analysis. We conducted a pilot study using the workflow to determine whether HDL protein glycoprofiles are altered in healthy human participants in response to dietary glycan supplementation.
Keyphrases
- high density
- high resolution
- endothelial cells
- small molecule
- mass spectrometry
- induced pluripotent stem cells
- protein protein
- liquid chromatography
- cancer therapy
- study protocol
- pluripotent stem cells
- high throughput
- randomized controlled trial
- high resolution mass spectrometry
- tandem mass spectrometry
- amino acid
- ultra high performance liquid chromatography
- binding protein
- cell surface
- single cell
- simultaneous determination