Evaluation and Limitations of the Novel Chemiluminescent Enzyme Immunoassay Technique for Measuring Total Tau Protein in the Cerebrospinal Fluid of Patients with Human Prion Disease: A 10-Year Prospective Study (2011-2020).
Kong WeijieToshiaki NonakaKatsuya SatoPublished in: Diagnostics (Basel, Switzerland) (2024)
Our findings indicate that CLEA outperforms ELISA in terms of diurnal reproducibility, storage stability, and freeze-thaw effects. However, ELISA demonstrated superior performance in the dilution assay. Therefore, it is imperative to develop innovative approaches for the dilution of biomarker samples for CLEA measurements during clinical trials.
Keyphrases
- cerebrospinal fluid
- clinical trial
- liquid chromatography tandem mass spectrometry
- endothelial cells
- liquid chromatography
- gas chromatography
- induced pluripotent stem cells
- high throughput
- monoclonal antibody
- pluripotent stem cells
- protein protein
- mass spectrometry
- randomized controlled trial
- amino acid
- sensitive detection
- binding protein
- simultaneous determination
- high resolution
- study protocol