Lactobacillus rhamnosus LRa05 Ameliorate Hyperglycemia through a Regulating Glucagon-Mediated Signaling Pathway and Gut Microbiota in Type 2 Diabetic Mice.
Tao WuYongli ZhangWen LiYunjiao ZhaoHairong LongEsther Mwizerwa MuhindoRui LiuWenjie SuiQian LiMin ZhangPublished in: Journal of agricultural and food chemistry (2021)
In this study, we aimed to explore the antidiabetic effects of Lactobacillus rhamnosus LRa05 on glucose metabolism and gut microbiota in type 2 diabetes mellitus (T2DM) mice. Our data indicated that the fasting blood glucose levels were reduced by 53.5% after treatment with LRa05 at a dose of 109 CFU·day-1. Meanwhile, LRa05 attenuated insulin resistance, relieved hepatic oxidative stress, and alleviated metabolic lipopolysaccharide-related inflammation in T2DM mice. LRa05 promoted the expression of glucose transporter 2, while it inhibited the expression of glucagon receptor, glucose-6-phosphatase, cellular adenosine-3'-5'-cyclic monophosphate-dependent protein kinase, and phosphoenolpyruvate carboxykinase in diabetic mice. Meanwhile, LRa05 reshaped gut microbiota, resulting in increased short-chain fatty acid bacteria (Alloprevotella and Bacteroides) and decreased proinflammatory bacteria (Odoribacter and Mucispirillum). Thus, LRa05 may be used as a functional food supplement for modulating the disorder glucose metabolism and gut microbiota in T2DM.
Keyphrases
- blood glucose
- glycemic control
- oxidative stress
- insulin resistance
- protein kinase
- signaling pathway
- poor prognosis
- high fat diet induced
- type diabetes
- fatty acid
- blood pressure
- binding protein
- inflammatory response
- dna damage
- metabolic syndrome
- electronic health record
- ischemia reperfusion injury
- diabetic rats
- pi k akt
- induced apoptosis
- skeletal muscle
- risk assessment
- big data
- long non coding rna
- lps induced
- polycystic ovary syndrome
- endoplasmic reticulum stress
- data analysis
- heat shock protein