CircEZH2 Regulates Milk Fat Metabolism through miR-378b Sponge Activity.
Dongyang WangZhengjiang ZhaoYiru ShiJunyi LuoTing ChenQianyun XiYongliang ZhangJia Jie SunPublished in: Animals : an open access journal from MDPI (2022)
In this study, we evaluated the roles of heat-induced circEZH2 in the regulation of milk fat metabolism. CircEZH2 overexpression increased HC11 cell proliferation and decreased apoptosis. These changes were accompanied by increased expression of proliferation marker proteins (PCNA, Cyclin D, and Cyclin E) and the anti-apoptotic protein Bcl2, while expression of the pro-apoptotic proteins Bax and cleaved-caspase was reduced. SiRNA-mediated silencing of EZH2 in HC11 cells had the opposite effects. CircEZH2 overexpression promoted the uptake of a fluorescent fatty acid (Bodipy) as well as expression of the fatty acid transport-related protein CD36, lipolysis-related protein LPL, and unsaturated fatty acid metabolism-related proteins FADS1 and SCD1. Dual luciferase reporter assays verified the targeting relationship of the two ceRNA networks, circEZH2-miR378b-LPL and circEZH2-miR378b-CD36. This information provides further clarification of the role of circRNAs in milk fat regulation in addition to a theoretical basis for alleviating the effects of heat stress on milk production by dairy cows.
Keyphrases
- fatty acid
- cell cycle arrest
- cell death
- heat stress
- cell proliferation
- poor prognosis
- induced apoptosis
- adipose tissue
- cell cycle
- long non coding rna
- dairy cows
- binding protein
- pi k akt
- endoplasmic reticulum stress
- oxidative stress
- signaling pathway
- transcription factor
- cancer therapy
- anti inflammatory
- quantum dots
- drug delivery
- heat shock
- health information
- single cell
- protein protein
- amino acid
- small molecule