PDR9 allelic variation and MYB63 modulate nutrient-dependent coumarin homeostasis in Arabidopsis.
Megan DeLooseHuikyong ChoNadia BouainIlyeong ChoiChanakan Prom-U-ThaiZaigham ShahzadLuqing ZhengHatem RouachedPublished in: The Plant journal : for cell and molecular biology (2024)
Plant roots release phytochemicals into the soil environment to influence nutrient availability and uptake. Arabidopsis thaliana roots release phenylpropanoid coumarins in response to iron (Fe) deficiency, likely to enhance Fe uptake and improve plant health. This response requires sufficient phosphorus (P) in the root environment. Nonetheless, the regulatory interplay influencing coumarin production under varying availabilities of Fe and P is not known. Through genome-wide association studies, we have pinpointed the influence of the ABC transporter G family member, PDR9, on coumarin accumulation and trafficking (homeostasis) under combined Fe and P deficiency. We show that genetic variation in the promoter of PDR9 regulates its expression in a manner associated with coumarin production. Furthermore, we find that MYB63 transcription factor controls dedicated coumarin production by regulating both COUMARIN SYNTHASE (COSY) and FERULOYL-CoA 6'-HYDROXYLASE 1 (F6'H1) expression while orchestrating secretion through PDR9 genes under Fe and P combined deficiency. This integrated approach illuminates the intricate connections between nutrient signaling pathways in coumarin response mechanisms.
Keyphrases
- transcription factor
- fluorescent probe
- poor prognosis
- arabidopsis thaliana
- metal organic framework
- healthcare
- dna binding
- genome wide identification
- dna methylation
- genome wide association
- signaling pathway
- public health
- gene expression
- aqueous solution
- replacement therapy
- binding protein
- genome wide
- visible light
- plant growth
- fatty acid
- long non coding rna
- heavy metals
- health information
- oxidative stress
- social media