A Modified Protocol for the Isolation, Culture, and Characterization of Human Smooth Muscle Cells from the Umbilical Cord.

Asmaa Q IbrahimMohammed S AbdullahMamoun Ahram Shtaywy S Abdalla

Published in: Methods and protocols (2023)

The protocol described herein for the isolation of VSMCs from human umbilical cords is easy and is time- and cost-efficient. Isolated cells are useful models for understanding the mechanisms underlying many pathophysiological conditions.

Keyphrases

umbilical cord
mesenchymal stem cells
induced apoptosis
randomized controlled trial
endothelial cells
cell cycle arrest
induced pluripotent stem cells
endoplasmic reticulum stress
signaling pathway
pluripotent stem cells
vascular smooth muscle cells
cell death
cell proliferation