Genome-wide CRISPRi Screen in Human iNeurons to Identify Novel Focal Cortical Dysplasia Genes.
Andrew M TidballJinghui LuoJ Clayton WalkerTaylor N TaklaGemma L CarvillJack M ParentPublished in: bioRxiv : the preprint server for biology (2023)
genetic screen in human neurons using an FCD biomarker, uncovering genes that increase neuronal mTOR signaling when their expression is lost. Two candidate genes were mutated in patient FCD brain tissues in a recent study, supporting a causative role for these genes in FCD. Our work suggests gene candidates for somatic variant analysis in FCD tissue and indicates added value for genetic screening in neurons over cell lines.
Keyphrases
- genome wide
- copy number
- dna methylation
- endothelial cells
- genome wide identification
- gene expression
- spinal cord
- high throughput
- pluripotent stem cells
- induced pluripotent stem cells
- poor prognosis
- cell proliferation
- cerebral ischemia
- multiple sclerosis
- white matter
- spinal cord injury
- transcription factor
- bioinformatics analysis
- genome wide analysis