CTCF orchestrates the germinal centre transcriptional program and prevents premature plasma cell differentiation.
Arantxa Pérez-GarcíaEster Marina-ZárateÁngel F Álvarez-PradoJose M LigosNiels GaljartAlmudena R RamiroPublished in: Nature communications (2017)
In germinal centres (GC) mature B cells undergo intense proliferation and immunoglobulin gene modification before they differentiate into memory B cells or long-lived plasma cells (PC). GC B-cell-to-PC transition involves a major transcriptional switch that promotes a halt in cell proliferation and the production of secreted immunoglobulins. Here we show that the CCCTC-binding factor (CTCF) is required for the GC reaction in vivo, whereas in vitro the requirement for CTCF is not universal and instead depends on the pathways used for B-cell activation. CTCF maintains the GC transcriptional programme, allows a high proliferation rate, and represses the expression of Blimp-1, the master regulator of PC differentiation. Restoration of Blimp-1 levels partially rescues the proliferation defect of CTCF-deficient B cells. Thus, our data reveal an essential function of CTCF in maintaining the GC transcriptional programme and preventing premature PC differentiation.
Keyphrases
- transcription factor
- gas chromatography
- gene expression
- signaling pathway
- cell proliferation
- induced apoptosis
- heat shock
- poor prognosis
- binding protein
- big data
- mouse model
- mass spectrometry
- machine learning
- quality improvement
- randomized controlled trial
- single cell
- clinical trial
- pi k akt
- dna binding
- electronic health record
- endoplasmic reticulum stress
- data analysis
- deep learning