Delivery of Plasmid DNA by Ionizable Lipid Nanoparticles to Induce CAR Expression in T Cells.
Pedro Henrique Dias Moura PrazeresHeloísa Athaydes Seabra FerreiraPedro Augusto Carvalho CostaWalison da SilvaMarco Túllio AlvesMarshall PadillaAjay ThatteAnderson Kenedy SantosAnderson Oliveira LoboAdriano de Paula SabinoHelen Lima Del PuertoMichael J MitchellPedro Pires Goulart GuimaraesPublished in: International journal of nanomedicine (2023)
Collectively, our results show that LNP-mediated delivery of pDNA is a suitable method to engineer human T cells to express CAR, which holds promise for improving the production methods and broader application of this therapy in the future.